TAfinder
Input Your Sequence
Example: example.fas
The coordinate in genome of your First sequence is ..
The coordinate in genome of your Second sequence is ..
If the coordinates of your input sequences are not available, please set the first residue in your first sequence as 1st, calculate and fill the rest.
Example : seq1: 1..396 , seq2: 398..694

Example: Escherichia coli ED1a chromosome [NC_011745]

Example: NC_011745


Or 4. Upload Your Sequence
Please:
Upload an FAA File (Required)
Example: Example.faa
Upload a PTT File (Required)
Example: Example.ptt
Or if you don't have FAA file or PTT file, you can upload the corresponding GenBank File to Generate the File Required
Example: Example1.gb without CDS translated sequence
Example2.gb with CDS translated sequence
Instruction:
We Need Both an FAA file and a PTT file;
GenBank(full) file with translated sequence such as Example2.gb can be used to generate BOTH of these two file

BUT GenBank file without translated sequence such as Example1.gb can only generate PTT file.

So in this case the corresponding FAA file MUST be uploaded.


Example: Example.fas

Instruction: Please use CDSeasy to quick annotate your complete sequence [with the "CDSeasy includes" option of "ONLY Gene finding (Prodigal)"],
and then submit the generated .gbk file into the above section "4.1 Upload an Annotated Genome Sequence".
Example: Example.fas


Instruction: Please use CDSeasy to quick annotate your Contig/Scaffold sequences [with the "CDSeasy includes" option of "ONLY Gene finding (Prodigal)"],
and then submit the generated .gbk file into the above section "4.1 Upload an Annotated Genome Sequence".

Upload Your File (FASTA/PTT/GenBank format) Here
Set Parameters (Optional)

(1) E-value for BLAST = (0.000001~0.01)
(2) E-value for HMMer = (0.001~1)
(3) Maximum length of potential toxin/antitoxin = (30~500 aa)
(4) Maximum distance(or overlap) between potential toxin and antitoxin = (-50~300 nt) (overlap_gap nt, in this case, it means gap between 20 nt overlap and 150 nt. )

Please click "Run" after the required file has been uploaded.

Retrieve Results
Input your job ID